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In the context of dermo-cosmetic research, LC-OCT enables precise evaluation of the effects of topical formulations on skin hydration and barrier integrity. When combined with advanced AI algorithms, it provides robust, quantitative, and reproducible metrics of skin microarchitecture and composition in vivo over time. These parameters serve as objective and reliable endpoints for product development and clinical studies, making LC-OCT a powerful tool for understanding skin physiology and evaluating the efficacy of hydration-focused cosmetic treatments.